We describe here for the first time DNA elution from buccal cells collected on Whatman FTA Classic Cards using a modified methanol fixation method ( 5, 8). Notably, the effects of different fixation techniques on chromatin ultrastructure and DNA extraction have shown cell morphology to be compromised with an increased yield of genomic DNA using a methanol-based fixative ( 6, 7). Methanol fixation during DNA extraction has been used previously for blood samples stored on Guthrie cards ( 5). Another issue with this is the relatively fast consumption of biological sample. However, the time-consuming sample punch process is a known rate-limiting step, which is exacerbated if a particular analysis requires multiple sample preparations ( 4) ( ).
With improved stability and lack of discomfort during collection, they are becoming the sample of choice allowing easy collection in any field situation.
Theoretically, buccal cells have the potential to generate more DNA product per volume than blood. The protocols do not adjust reaction volume or PCR conditions to the presence of the disc ( It has been found that the use of saliva samples is a good alternative to blood samples ( 2) to obtain genomic DNA of high quality, and that their use increases the response rate considerably in epidemiologic studies ( 3). WB120204), dried, and the entire sample disc then incorporated directly into the PCR amplification reaction. Whatman standard protocols require the disc to be washed in FTA purification reagent (Cat. For analysis, a small disc is punched from the dried sample area. FTA technology prevents the degradation of genomic DNA at room temperature, and PCR amplification of viable DNA after long-term archiving is possible ( 1). WB100032) are used to painlessly collect loose inner-cheek (buccal) cells, including those that are floating in saliva. WB120205 Whatman International Ltd, Piscataway, NJ, USA) combined with sterile foam tipped applicators (Cat.
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